Calculate enzyme activity (U) and specific activity (U/mg) from absorbance, volume, and more.
Enter your experimental data to determine enzyme activity and, if desired, specific activity. All calculations are based on standard enzyme kinetics formulas.
See how to calculate enzyme activity with real-world data.
Calculate enzyme activity from absorbance change, total volume, extinction coefficient, path length, enzyme volume, and time.
ΔA (Change in Absorbance): 0.25
Total Volume (mL): 3 mL
Extinction Coefficient (M⁻¹cm⁻¹): 6220 M⁻¹cm⁻¹
Path Length (cm): 1 cm
Enzyme Volume (mL): 0.1 mL
Time (min): 5 min
Calculate both enzyme activity and specific activity with protein amount provided.
ΔA (Change in Absorbance): 0.18
Total Volume (mL): 2.5 mL
Extinction Coefficient (M⁻¹cm⁻¹): 5400 M⁻¹cm⁻¹
Path Length (cm): 1 cm
Enzyme Volume (mL): 0.08 mL
Time (min): 4 min
Protein Amount (mg): 0.4 mg
A case with high absorbance change and short reaction time.
ΔA (Change in Absorbance): 0.45
Total Volume (mL): 4 mL
Extinction Coefficient (M⁻¹cm⁻¹): 7000 M⁻¹cm⁻¹
Path Length (cm): 1 cm
Enzyme Volume (mL): 0.12 mL
Time (min): 2 min
A case with low absorbance change and longer reaction time.
ΔA (Change in Absorbance): 0.05
Total Volume (mL): 3.5 mL
Extinction Coefficient (M⁻¹cm⁻¹): 6000 M⁻¹cm⁻¹
Path Length (cm): 1 cm
Enzyme Volume (mL): 0.1 mL
Time (min): 10 min