Accurate FENA calculation requires proper specimen collection, precise laboratory measurements, and careful interpretation of results. Follow this comprehensive methodology to ensure your FENA assessment provides reliable clinical information for patient management decisions.
1. Laboratory Data Collection and Preparation
Begin with simultaneous collection of blood and urine samples to ensure accurate FENA calculation. Blood samples should be collected in appropriate tubes and processed according to laboratory protocols. Urine samples should be spot urine collections or timed collections as specified by your laboratory. Ensure proper labeling and timely transport to the laboratory. For accurate results, avoid diuretic use for 24-48 hours before testing, as diuretics can significantly affect sodium excretion patterns and invalidate FENA interpretation.
2. Precise Data Entry and Validation
Enter urine sodium concentration in mEq/L (normal range 20-200 mEq/L), serum sodium in mEq/L (normal range 135-145 mEq/L), urine creatinine in mg/dL (varies with hydration status), and serum creatinine in mg/dL (indicates kidney function). The calculator automatically validates realistic ranges and provides specific error messages for invalid inputs. Pay attention to units - ensure all values are in the correct units before calculation. For patients with very low or very high values, double-check the measurements as extreme values may indicate laboratory error or critical clinical conditions.
3. Comprehensive Result Analysis
The calculator provides the FENA percentage and clinical interpretation. FENA <1% strongly suggests prerenal azotemia, while FENA >2% indicates intrinsic kidney injury. Values between 1-2% may represent normal function or early kidney injury requiring clinical correlation. The Kidney Injury Classification provides specific guidance on the type of kidney injury, and Clinical Recommendations offer evidence-based management suggestions. Consider the clinical context - FENA interpretation may be affected by diuretic use, chronic kidney disease, or specific medications.
4. Clinical Application and Treatment Planning
Use FENA results to guide treatment decisions. For prerenal azotemia (FENA <1%), focus on volume resuscitation, blood pressure optimization, and addressing the underlying cause of decreased kidney perfusion. For intrinsic kidney injury (FENA >2%), consider nephrology consultation, medication adjustments, and specific treatments for the underlying cause. Monitor FENA trends over time to assess response to treatment. Remember that FENA is one component of a comprehensive kidney evaluation - always correlate with clinical findings, other laboratory tests, and imaging studies.