Accurate SAAG calculation requires proper sample collection, precise laboratory measurement, and careful interpretation of results. Follow this systematic approach to ensure reliable diagnostic information.
1. Sample Collection and Processing
Serum albumin is measured from a venous blood sample collected in a standard blood collection tube. The sample should be processed within 2-4 hours to prevent protein degradation. Ascites albumin is measured from fluid collected during diagnostic or therapeutic paracentesis. The ascitic fluid should be collected in a sterile container and sent immediately to the laboratory for analysis. Both samples should be analyzed using the same laboratory method (typically bromcresol green or immunoturbidimetric assays) to ensure consistency.
2. Laboratory Measurement Standards
Albumin measurement should be performed using standardized laboratory methods with appropriate quality controls. The bromcresol green method is most commonly used and provides reliable results. Laboratories should maintain calibration standards and participate in proficiency testing programs. Results should be reported to at least one decimal place (e.g., 3.5 g/dL) to ensure sufficient precision for SAAG calculation.
3. Calculation and Data Entry
Enter the serum albumin value in the first field, ensuring you use the correct units (g/dL). Enter the ascites albumin value in the second field, also in g/dL. The calculator will automatically compute the SAAG by subtracting the ascites albumin from the serum albumin. Double-check your input values before calculation, as small errors can significantly affect the interpretation.
4. Clinical Interpretation
Interpret the SAAG result in the context of the patient's clinical presentation. SAAG ≥ 1.1 g/dL strongly suggests portal hypertension, while SAAG < 1.1 g/dL indicates other causes. However, always consider additional factors such as the patient's medical history, physical examination findings, and other laboratory results. In some cases, additional diagnostic tests may be necessary to confirm the diagnosis.